Advansta Inc.

Category: Content
Type: Blog Article

Generated 3 weeks ago

New blog articles detected

  • The Ultimate SDS-PAGE Gel Making Tips and Tricks

    Prepare yourself for a true story. After graduation, a newly minted postdoc left our molecular biology lab to work in a clinical science lab in Germany. He developed a reputation in our lab for his SDS-PAGE gels, which gave the sharpest bands on Westerns. He passed along this protocol to some of the medical students... Read more » The post The Ultimate SDS-PAGE Gel Making Tips and Tricks appeare...

Advansta Inc.

Category: Content
Type: Blog Article

Generated 1 month ago

New blog articles detected

  • How to get rid of your Western smile

    Sometimes your Western blot bands are not straight but  “smile” – the bands look more like crescent moons than straight lines. And, unlike your smile, a smiling Western is not a good thing. Band distortion makes it harder to determine the molecular weight of the target protein, complicates band shift detection and makes densitometry challenging.... Read more » The post How to get rid of your West...

  • Protein Transfer Method for All Seasons – Wet, Semidry, Dry

    Everybody has their favorite method of transferring proteins to the membrane and people reluctant to change them. There’s always “if it ain’t broken, don’t fix it” wisdom. But one day you will realize that your trusted tank irrevocably broke down. And on that black one day, it’s better to know what else is available and... Read more » The post Protein Transfer Method for All Seasons – Wet, Semidr...

  • My Fair Lady ELISABright™ and Logistic Regression

    Let’s say you have worked very hard and you have developed a perfect ELISA assay. Your plate is coated to perfection, non-specific binding is blocked and all samples have been properly diluted to fit your assay’s limits. Don’t let the wrong curve fit ruin your day! Choosing the Logistic Regression Model Whether you prefer direct... Read more » The post My Fair Lady ELISABright™ and Logistic Regre...

  • What is block doing in blots & ELISAs and how to improve it

    In many labs, blocks for Western Blot, Dot Blot, and ELISA are “one size fits all” – one buffer and one blocking agent for any antibody. If the combination doesn’t work, people would rather give up than try changing the mix. So why do we put a milky buffer on our membranes? Consider the block... Read more » The post What is block doing in blots & ELISAs and how to improve it appeared first on Adv...

Advansta Inc.

Category: Content
Type: Blog Article

Generated 6 months ago

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